In vivo bioluminescent calcium imaging: still a long way to go
Published in CNS2024, 2024
This poster was presented in Suzhou CNS2024.
Abstract
Motivation Bioluminescence imaging avoids excitation light, overcoming photobleaching and phototoxicity of traditional fluorescence imaging. It promises imperceptible, long-term neural activity imaging in living animals.
Questions
- Dim signal in vivo
- Bad bioluminescent calcium sensors
- Substrates struggle to enter animal cells
Approaches
- Screening brighter substrates
- Develop novel sensor with high performance
- Engineering animal for autoluminescence
Conclusions We aim to enhance bioluminescent calcium imaging tools, establishing them as a standard in neurobiology labs.
Poster
